Comparison of the different teratogenic effects of three commerical samples of trypan blue.

Comparison of the Different Teratogenic Effects of Three Commercial Samples of Trypan Blue

Comparison of MTT and Trypan Blue methods in determining the survival of Vero cell line in HSV-1 infection

Abstract Background and Objectives Evaluation of cell survival is important in cellular studies and the result is decisive for the application of the test substance. Different methods are used to evaluate cell survival and the correlation between methods is important. Here, Vero cell line was used to evaluate the effects of HSV-1 virus on cell survival. Cell survival after HSV-1 infection usin...

Comparison of MTT, trypan blue, and clonogenic assay, to determine the viability in human anaplastic thyroid cancer cell line

Background: Evaluation of cell viability is momentous in pharmacologic and oncological research. Cell viability evaluation determines cell sensitivity and consequently treatment outcome. Various methods are available to determine cell survival. Each of these methods evaluates different endpoints. Accordingly, determining the correlation between these methods is important. In this study, in orde...

The relationship of chemical structure to teratogenic activity among bisazo dyes: a re-evaluation.

Although the potency of trypan blue as a teratogenic agent in rodents has been known for almost twenty years the mechanism of its action remains obscure. The dye produces a variety of biological effects (see review by Beck & Lloyd, 1966) and most of these have at some time been suggested as possibly relevant to its teratogenicity. The investigation of such correlations would clearly be assisted...

Further observations on the teratogenic action of the thyroid stimulating hormone.

Pregnant Wistar Albino rats were injected intraperitoneally with the thyroid stimulating hormone (TSH), obtained from several different suppliers, and with the dye trypan blue. Twenty USP units of TSH were injected on days 7 to 10 of pregnancy, one quarter of the total dose each day. Trypan blue was injected as a single subteratogenic dose (0.6 mg/100 gm maternal body weight) on day 8. Autopsy ...